刘畅（liuchang4@genomics.cn），博士。主要研究方向为细胞组学技术开发以及胚胎发育和疾病的多组学研究，曾主导包括国自然青年基金在内的 2 项科研项目，近 5 年发表 SCI 论文 10 篇，以第一及通讯作者（含共同）发表在Developmental Cell，Nature Communications，Scientific Data, Journal of Genetics and Genomics 等，申请单细胞组学技术专利软著 3 项。在技术开发方向，作为主要负责人之一参与基于 DNB 芯片的细胞测序技术、基于单分子长度长测序的单细胞全长测序技术的开发，相关方法学文章已经预印或在 Science 返修。在科学研究方面，代表性成果为利用 Stereo-seq 测序技术绘制了斑马鱼胚胎早期发育的时空图谱，以封面故事发表在 Developmental Cell，后续工作正在包括 PNAS 在内的多篇杂志返修。
研究方向：细胞组学技术开发及在发育中的研究应用
现任岗位：华大研究院 细胞所 生物技术副研究员
教育背景：中国科学院大学 生物物理研究所 神经生物学 理学博士
参与项目：
1.2018. 1– 2019.12 中央高校基本科研业务费专项资金青年教师资助基金项目，2042018kf0083，RECS1 在急性肺损伤中的作用及其机制研究，6 万，已结题。
2.2017. 1– 2019.12 国家自然科学基金青年科学基金项目，31601181，基于 Single Cell RNA-seq 的斑马鱼神经干细胞不对称分裂调控机制研究，20 万，已结题。
3.2025. 1– 2029.12 国家重点研发计划,SQ2024YFA1700017，面向光合微藻细胞工厂创制的智能化多组学精细解析技术，2000 万，进行中。
4.2024. 1– 2026.12 杭州市领军型创新创业团队,TD2023003， 高通量单细胞多组学生物大分子解析平台创新团队，250 万，进行中。
5.2024. 4– 2027.3 杭州重点研发计划，2024SZD0128，单细胞多组学解析技术及其应用，300 万，进行中。
论文:
1.Sha Liao*, Xiaoxi Zhou*, Chuanyu Liu*, Chang Liu*, Shijie Hao*, Hongyu Luo*,..., Longqi Liu#, Ao Chen# & Xun Xu#, Stereo-cell: spatial enhanced resolution single-cell sequencing with high-density DNA nanoball-patterned arrays, Science. Under Revision.
2.Chang Liu, Xuerong Li, Qinan Hu, Zihan Jia, Qing Ye, Xianzhe Wang, Kaichen Zhao, Longqi Liu#, Mingyue Wang#, Decoding the blueprints of embryo development with single-cell and spatial omics, Seminars in Cell and Developmental Biology. Revision Submitted to Journal.
3.Fangbin Chen*, Yue Zhang*, Xiaoming Wang*, Zhaohua Deng*,...,Pengfei Pang#, Wei Ge#, Chang Liu#, Hong Shan# and Huanhuan He#, Organ-differential Regulation of Vascular Development by DEAD-box Helicase 24, PNAS. Under Revision.
4.Ruolin Zhang*, Sicheng Guo*, Jie Zhou*, ..., Chang Liu# and Zhiqiang Dong#, Monitoring of single-nucleus chromatin landscape of ischemic stroke in mouse cerebral cortex across time, Scientific Data. 2024, Accepted.
5.Jie Zhou, Xueqian Yang, Xiumei Lin, Kaichen Zhao, Xue Wang, Zhiqiang Dong, Chuanyu Liu#, and Chang Liu#, A protocol to profile snATAC-seq datasets and motif enrichment analysis during zebrafish early embryogenesis, Star Protocol. 2024, Accepted.
6.Xiumei Lin*, Xue Wang*, Chang Liu*, Chuanyu Liu*, Tao Zeng*, Ziqi Yuan*, ..., Yuliang Dong#, Xun Xu#, Daji Luo#, Ying Gu#, Longqi Liu#, Zhiqiang Dong#, Liang Chen#, Deciphering the Transcript Heterogeneity and Alternative Splicing during the Early Zebrafish Cell Fate Determination, BioRxiv. 2024.
7.Xiumei Lin*, Xueqian Yang*, Chuan Chen*, ..., Zhiqiang Dong#, Chuanyu Liu#, Longqi Liu#, Chang Liu#, Single-nucleus chromatin landscapes during zebrafish early embryogenesis, Scientific Data. 2023,10: 464.
8.Chang Liu*, Rui Li*, Young Li*, ..., Xun Xu#, Zhiqiang Dong#, Longqi Liu#, Spatiotemporal mapping of gene expression landscapes and developmental trajectories during zebrafifish embryogenesis, Developmental Cell. 2022, 57: 1284– 1298.
9.Jinghan Sun*, Chang Liu*, Xiaobing Bai*, Xiaoting Li, Jingyun Li, Zhiping Zhang, Yunpeng Zhang, Jing Guo, and Yan Li, Drosophila FIT is a proteinspecific satiety hormone essential for feeding control. Nature Communications. 2017, 8: p. 14161.
10.Chang Liu, Xiaobing Bai, Jinghan Sun, Xiaofan Zhang, Yan Li, Behavioral Switch of Food Preference upon Sugar Deficiency Is Regulated by GPCRs in Drosophila, Journal of Genetics and Genomics. 2015, 42: 409-412.
其他论文：
11.Shijie Hao*, Xiaojia Zhu*, Zhi Huang*, Qiangian Yang*, HeanLiu*, Yan Wu*, Yafeng Zhan*, Yu Dong*, Chao Li*,..., Chang Liu, ..., De Zeeuwe#, Zhiming Shen#, Zhiyong Liu#, LongqiLiul#, Shiping Liu#, Yidi Sun#, Cirong Liu#. Crossspecies single-cell spatial transcriptomic atlases of the cerebellar cortex. Science. 2024, on line.
12.Baihua Wu, Xinyi Shentu, Haitao Nan, Pengcheng Guo, Shijie Hao, Jiangshan Xu, Shuncheng Shangguan, Lei Cui, Jin Cen, Qiuting Deng, Yan Wu, Chang Liu,...A spatiotemporal atlas of cholestatic injury and repair in mice. Nature Genetics. 2024 volume 56, pages938– 952 (2024).
13.Qiuting Deng, Shengpeng Wang, Zijie Huang, Qing Lan, Guangyao Lai, Jiangshan Xu, Yue Yuan, Chang Liu, ..., Yingjie Luo, Longqi Liu, Chuanyu Liu, Single-cell chromatin accessibility profiling of cell-state-specific gene regulatory programs during mouse organogenesis. Frontiers in Neuroscience. 2023, doi: 10.3389/fnins.2023.1170355.
14.Peng-Cheng Guo, Jing Zuo, Ke-Ke Huang, Guang-Yao Lai, Xiao Zhang, Juan An, Jin-Xiu Li, Li Li, Liang Wu, Yi-Ting Lin, Dong-Ye Wang, JiangShan Xu, Shi-Jie Hao, Yang Wang, Rong-Hai Li, Wen Ma, Yu-Mo Song, Chang Liu, ..., Miguel A. Esteban, Yi-Wei Lai, Cell atlas of CCl4- induced progressive liver fibrosis reveals stage-specific responses. Zoological Research. 2023, 44(3): 451-466
15.Shi X, Ma W, Duan S, Shi Q, Wu S, Hao S, Dong G, Li J, Song Y, Chang Liu, ..., Liu C, Liu L. Single-cell transcriptional diversity of neonatal umbilical cord blood immune cells reveals neonatal immune tolerance. Biochem Biophys Res Commun. 2022, 608:14-22
16.Yin J, ZhaoY, Huang F, Yang Y, HuangY, Zhuang Z, Wang Y, Wang Z, Lin X, Zheng Y, Zhou W, Wang S, Xu Z, Ye B, Guo Y, Lei W, Li L, Tian J, Gan J, Wang H, Wang W, Ma P, Chang Liu, ..., Liu C, Yang X, Liu W, Single-cell transcriptome sequencing reveals the immune response and homeostasis mechanism following administration of BBIBP-CorV SARS-CoV- 2 inactivated vaccine. The Innovation. 2022, 44:2666-6758
专利和软著：
1.黎雪蓉， 刘畅，林秀妹，刘传宇，罗焜月，宋雨默，刘龙奇，基于液滴的 PCR 扩增、建库及测序方法, 2023-12-28, 美国, PCT/CN2023/139573
2.王雪，林秀妹， 刘畅，刘传宇，刘龙奇，基于基因表达趋势相似性分析软件，中国， 2024SR0580792
3.林秀妹，王雪，杨世晨， 刘畅，刘传宇，刘龙奇，基于局部比对算法拆分 barcode 流程软件，中国， 2024SR0960279
4.王雪，林秀妹， 刘畅，刘传宇，刘龙奇，基于机器学习自动注释 scATAC-seq 细胞群体的软件，中国， 2024SR1193384
