张海强（zhanghaiqiang@genomics.cn），深圳华大生命科学研究院生物技术副研究员。2015年于浙江大学获得硕士学位，2019年于香港中文大学医学院获得博士学位，具有10余年的高通量测序数据分析和生物信息算法开发经验，长期从事基于游离核酸的液体活检研究，致力于体液中循环游离DNA和RNA的生物学特性解析和其在疾病无创检测中的临床应用探索，包括癌症早筛，无创产前诊断和感染性疾病的诊治研究。近年来在Advanced Science, Trends in Microbiology, Clinical Chemistry，PNAS，NEJM等期刊发表SCI论文25篇，申请液体活检相关专利7项，获授权软件著作权15项。
研究方向：游离核酸与液体活检
现任岗位：生物技术副研究员
教育背景：博士，化学病理学，香港中文大学
研究摘要：
游离DNA (cell-free DNA, cfDNA) 是人体体液循环中游离于细胞外的DNA。人体外周血中cfDNA主要来源于细胞的凋亡和坏死，携带着丰富的组织特异性生理和病理信号。由于外周血采样的简便、无创、可重复取样等优点，cfDNA已逐渐成为一种新型无创分子标志物，基于血浆游离DNA的无创产前筛查和肿瘤液体活检两大技术在临床应用中发展迅速。近年来，本团队在母胎医学，感染，肿瘤，代谢性疾病和免疫疾病方向共开展超过15项无创标志物研究并取得多项成果，覆盖疾病早筛到诊治全周期，为疾病的临床诊疗提供重要指导。目前我们的研究方向主要有以下几个方面：
1.新型癌症早筛无创生物标志物研究
2.新型生物信息学算法开发
3.多组学技术在液体活检中的研究和临床应用
4.人工智能大模型在液体活检中的应用研究
论文:
1.Shaogang Li*, Yu Lin*, Fengxia Su*, Xintao Hu, Lingguo Li, Wei Yan, Yan Zhang, Min Zhuo, Ya Gao#, Xin Jin#, Haiqiang Zhang#. Comprehensive evaluation of the impact of whole-genome bisulfite sequencing (WGBS) on the fragmentomic characteristics of plasma cell-free DNA. Clinica Chimica Acta (2024): 120033.（通讯作者）
2.Ting Peng*, Haiqiang Zhang*, Lingguo Li*, Canhui Cao*, Miaochun Xu, Xiaojie Liu, Shitong Lin, Ping Wu, Tian Chu, Binghan Liu, Yashi Xu, Yan Zhang, Yeqin Wang, Jinjin Yu#, Wencheng Ding#, Xin Jin#, and Peng Wu#. Plasma cell-free DNA concentration and fragmentomes predict neoadjuvant chemotherapy response in cervical cancer patients. Advanced Science (2024) (共同一作)
3. Haiqiang Zhang*, Lingguo Li*, Yuxue Luo*, Fang Zheng*, Yan Zhang*, Rong Xie*, Rijing Ou, Yilin Chen, Yu Lin, Yeqin Wang, Yan Jin, Jinjin Xu, Ye Tao, Ruokai Qu, Wenwen Zhou, Yong Bai, Fanjun Cheng#, Xin Jin#. Fragmentomics of plasma mitochondrial and nuclear DNA inform prognosis in COVID-19 patients with critical symptoms. BMC Medical Genomics (2024) (第一作者)
4.Yuxuan Hu*, Yanping Zhao*#, Yan Zhang, Weijun Chen, Haiqiang Zhang#, Xin Jin#. Cell-free DNA: a promising biomarker in infectious diseases. Trends in Microbiology (2024). (通讯作者)
5.Haiqiang Zhang*, Longwei Qiao*, Xintao Hu, Chunhua Zhang, Yu Lin, Jingyu Zhao, Xiaojuan Wu, Xiaoyan Song, Hui Tang, Ying Xue, Yang Sun, Rijing Ou, Xinxin Wang, Yan Zhang, Xin Jin#, Ting Wang#. Detection of early-onset severe preeclampsia by cell-free DNA fragmentome. medRxiv (2024): 2024-03. (第一作者)
6.Huanhuan Zhu*, Yan Zhang*, Shuang Zeng*, Linxuan Li*, Rijing Ou*, Xinyi Zhang, Yu Lin, Ying Lin, Chuang Xu, Lin Wang, Guodan Zeng, Jingyu Zeng, Lingguo Li, Yongjian Jia, Yu Wang, Fei Luo, Meng Yang, Yuxuan Hu, Xiameizi Li, Han Xiao, Xun Xu, Jian Wang, Aifen Zhou#, Haiqiang Zhang#, Xin Jin#. cfGWAS reveal genetic basis of cell-free DNA features. medRxiv (2024), 2024-08. (通讯作者)
7.Xintao Hu*, Haiqiang Zhang*, Yeqin Wang*, Yu Lin*, Qiuyan Li, Lingguo Li, Guodan Zeng, Rijing Ou, Xinyu Cheng, Yan Zhang#, Xin Jin#. Effects of blood-processing protocols on cell-free DNA fragmentomics in plasma: Comparisons of one-and two-step centrifugations. Clinica Chimica Acta 560 (2024): 119729. (共同一作)
8.Yuxue Luo*, Haiqiang Zhang*, Lingguo Li, Yu Lin, Xinxin Wang, Wei Chen, Ye Tao, Rijing Ou, Wenwen Zhou, Fang Zheng, Yan Jin, Fanjun Cheng, Huanghuang Zhu, Yan Zhang, Xin Jin. Heat inactivation does not alter host plasma cell-free DNA characteristics in infectious disease research. Clinica Chimica Acta 553 (2024): 117751. (共同一作)
9.Chaolun Yu, Yu Lin, Yuxue Luo, Yun Guo, Zhiming Ye, Rijing Ou, Yan Zhang, Xinxin Wang, Ruokai Qu, Wenwen Zhou, Jie Li, Yong Bai, Xueqing Yu, Haiqiang Zhang#, Li Yan#, Xin Jin#. "The fragmentomic property of plasma cell-free DNA enables the non-invasive detection of diabetic nephropathy in patients with diabetes mellitus." Frontiers in Endocrinology 14 (2023). (通讯作者)
10.Xinping Chen*, Tao Wu*, Lingguo Li*, Yu Lin*, Zhichao Ma*, Jinjin Xu*, Hui Li*, Fanjun Cheng*, Ruoyan Chen*, Kun Sun, Yuxue Luo, Chen Zhang, Fang Chen, Jiao Wang, Tingyu Kuo, Xiaojuan Li, Chunyu Geng, Feng Lin, Chaojie Huang, Junjie Hu, Jianhua Yin, Ming Liu, Ye Tao, Jiye Zhang, Rijing Ou, Fang Zheng, Yan Jin, Huanming Yang, Jian Wang, Xun Xu, Shengmiao Fu#, Hongyan Jiang#, Xin Jin#, Haiqiang Zhang#. TSS Coverage Analysis in Plasma Cell-free DNA Reveals Disease Severity and Tissue Specificity of COVID-19 Patients. Frontiers in Genetics 12 (2021): 718. (通讯作者)
11.Mary-Jane L. Ma*, Haiqiang Zhang*, Peiyong Jiang*, Sarah T.K. Sin, W.K. Jacky Lam, Suk Hang Cheng, Wing-Shan Lee, Wanxia Gai, O.Y. Olivia Tse, Wenlei Peng, John Wong, Radha Raghupathy, Raymond S.M. Wong, Daljit Sahota, Tak Y. Leung, K.C. Allen Chan, Rossa W.K. Chiu, Y.M. Dennis Lo: Topologic Analysis of Plasma Mitochondrial DNA Reveals the Coexistence of Both Linear and Circular Molecules. Clinical chemistry, 2019, Clinical Chemistry. 2019.308122. （共同一作）
专利:
1.Lo, Y. M. D., Chiu, R. W. K., Chan, K. C., Jiang, P., Ji, L., Sin, T. K., Zhang, H., & Deng, J. Determining linear and circular forms of circulating nucleic acids: (2020). U.S. Patent Application No. 16/829,771.
2.Lo, Y. M. D., Chiu, R. W. K., Chan, K. C., Jiang, P., Lam, W. K., & Zhang, H. Nucleic acid rearrangement and integration analysis: (2020). U.S. Patent Application No. 16/456,354.
