李甫强(lifuqiang@genomics.cn)。2010年加入深圳华大生命科学研究院并从事癌症生物信息相关研究，基于多组学（基因组学、转录组学、表观组学、免疫组学、蛋白组学、单细胞组学、时空组学等）数据针对癌症的早期预测、异质性、发生发展、免疫微环境和分子分型等研究开展生物信息技术研发。在Nature、Cell、Advanced Materials、Nature Communications等国际学术期刊发表科研论文31篇，授权/受理发明专利13项，主持或作为课题骨干参与国家、省、市各级科研经费项目3项。
研究方向：癌症生物信息学
现任岗位：专项科学家，深圳华大基因股份有限公司-智惠医学研究院
教育背景：本科，生物医学工程，华中科技大学
参与项目:
1.肿瘤单细胞研究
2.肝癌多组织单细胞及游离核酸多组学研究
3.肺癌精准医学研究计划
4.基于细胞外游离DNA深度信息建立恶性肿瘤来源预测模型
5.肺癌基因编辑研究
6.癌症多组学大数据
7.癌症时空组学
8.癌症早期预测技术研发
9.基于多组学大数据的癌症免疫综合研究
10.慢病与健康大人群大数据
论文:
1.Boyang Cao, Huijuan Luo, Tian Luo, Kang Shao, Kui Wu, Sunil Kumar Sahu, Fuqiang Li*, Cong Lin*, The performance of whole genome bisulfite sequencing on DNBSEQ-Tx platform examined by different library preparation strategies. Heliyon, 2023. 9(6): E16571.
2.Luis Nunes#, Fuqiang Li#, Meizhen Wu#, Tian Luo#, Klara Hammarstrom, Emma Lundin, Ingrid Ljuslinder, Artur Mezheyeuski, Per-Henrik Edqvist, Anna Lofgren-Burstrom, Carl Zingmark, Sofia Edin, Chatarina Larsson, Lucy Mathot, Erik Osterman, Emerik Osterlund, Viktor Ljungstrom, Ines Neves, Nicole Yacoub, Unnur Gudnadottir, Helgi Birgisson, Malin Enblad, Fredrik Ponten, Richard Palmqvist, Mathias Uhlen, Kui Wu, Bengt Glimelius, Cong Lin, Tobias Sjoblom, Prognostic whole-genome and transcriptome signatures in colorectal cancers. medRxiv, 2023. (In Revision)
3.Xiafei Hong; Rui Jiang; Sitan Qiao; Wenze Wang; Bohui Yin; Fuqiang Li; Jingqiao Wang; Chao Ling; Xianze Wang; Yutong Wang; Yupei Zhao; Kui Wu; Wenming Wu, Integrated Multiomics Sequencing Reveals Precise Prognostic Stratification of Pancreatic Neuroendocrine Tumors. 2023 (In Revision)
4.Tao Tan; Dmitri Mouradov; Margaret Lee; Grace Gard; Yumiko Hirokawa; Shan Li; Cong Lin; Fuqiang Li; Huijuan Luo; Kui Wu; Michelle Palmieri; Evelyn Leong; Jordan Clarke; Anuratha Sakthianandeswaren; Helen Brasier; Jeanne Tie; Niall C. Tebbutt; Azim Jalali; Rachel Wong; Antony W. Burgess; Peter Gibbs; Oliver M. Sieber, Unified framework for patient-derived tumor organoid-based predictive testing of standard-of-care therapies in metastatic colorectal cancer. Cell Reports Medicine, 2023. 4(12): 101335.
5.Nannan Li, Lei Wang, Han Liang, Cong Lin, Ji Yi, Qin Yan, Huijuan Luo, Tian Luo, Liwei Zhang, Xiaojian Li, Kui Wu*, Fuqiang Li*, Ningchen Li*, Detecting and monitoring bladder cancer with exfoliated cells in urine. Front Oncol, 2022. 12: p. 986692.
6.Han Liang, Cong Lin, Yong Hou, Fuqiang Li*, Kui Wu*, Identification of the associated expression patterns as potential predictive markers for cancer prognosis. bioRxiv, 2020. (Preprint)
7.Han Liang, Jianlian Deng, Tian Luo, Huijuan Luo, Xuan Wu, Yiwang Ye, Shubin Wang, Fuqiang Li, Kui Wu, Cong Lin, Chromatin Accessibility Reveals Potential Prognostic Value of the Peak Set Associated with Smoking History in Patients with Lung Adenocarcinoma. bioRxiv, 2020. (Preprint)
8.Jianlian Deng; Fuqiang Li#; Guoyun Xie; Changzheng Wang; Kui Wu; Cong Lin; Huanming Yang, Identification of diagnostic and prognostic markers in biliary tract cancers with integrated genomic profiling. (In Revision)
9.Yuanyuan Ma, Bin Kang, Shaolei Li, Guoyun Xie, Jiwang Bi, Fuqiang Li, Guo An, Bing Liu, Jing Li, Yue Shen, Xun Xu, Huanming Yang, Yue Yang, Ying Gu, Nan Wu, CRISPR-mediated MECOM depletion retards tumor growth by reducing cancer stem cell properties in lung squamous cell carcinoma. Mol Ther, 2022. 30(11): p. 3341-3357.
10.Zhang B, Liang H, Liu W, Zhou X, Qiao S, Li F, Tian P, Li C, Ma Y, Zhang H, Zhang Z, Nanjo S, Russo A, Puig-Butillé JA, Wu K, Wang C, Zhao X, Yue D. A novel approach for the non-invasive diagnosis of pulmonary nodules using low-depth whole-genome sequencing of cell-free DNA. Transl Lung Cancer Res 2022;11(10):2094-2110. doi: 10.21037/tlcr-22-647
11.Xi Chen, Sitan Qiao, Yuanyuan Zhao, Xinlan Zhou, Hongyun Zhao, Kui Wu, Wenfeng Fang, Yan Huang, Fuqiang Li, Yunpeng Yang, Liyun Xiao, Shaodong Hong, Shida Zhu, Yuxiang Ma, Yong Hou, Yanhui Chen, Yaxiong Zhang, Xin Zhao, Li Zhang, Multi-region and multi-omics analysis reveal high heterogeneity and immunosuppressive feature of microenvironment in EGFR mutant lung adenocarcinoma. (In Revision)
12.Yumiko Hirokawa, Jordan Clarke, Michelle Palmieri, Tao Tan, Dmitri Mouradov, Shan Li, Cong Lin, Fuqiang Li, Huijuan Luo, Kui Wu, Maree Faux, Chin Wee Tan, Margaret Lee, Grace Gard, Peter Gibbs, Antony W. Burgess & Oliver M. Sieber, Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine. Commun Biol, 2021. 4(1): p. 1067.
13.Liang H#, Li F#*, Qiao S, Zhou X, Xie G, Zhao X, Zhang Y*, Wu K*. Whole-genome sequencing of cell-free DNA yields genome-wide read distribution patterns to track tissue of origin in cancer patients. Clinical and Translational Medicine, 2020, 10(6): e177.
14.Huang, Ao#; Zhao, Xin#; Yang, Xin-Rong#; Li, Fu-Qiang#; Zhou, Xin-Lan; Wu, Kui; Zhang, Xin; Sun, Qi-Man; Cao, Ya; Zhu, Hong-Mei; Wang, Xiang-Dong; Yang, Huan-Ming; Wang, Jian……, Circumventing intratumoral heterogeneity to identify potential therapeutic targets in hepatocellular carcinoma., Journal of Hepatology, 67(2), pp 293-301, 2017/8.
15.Xiao, Dakai#; Li, Fuqiang#; Pan, Hui; Liang, Han; Wu, Kui; He, Jianxing, Integrative analysis of genomic sequencing data reveals higher prevalence of LRP1B mutations in lung adenocarcinoma patients with COPD., Scientific Reports, 7(1), p 2121, 2017/5/18.
16.Wu, Kui#; Zhang, Xin#; Li, Fuqiang#; Xiao, Dakai#; Hou, Yong#; Zhu, Shida#; Liu, Dongbing; Ye, Xiaofei; Ye, Mingzhi; Yang, Jie; Shao, Libin; Pan, Hui; Lu, Na; Yu, Yuan; Liu, Liping; Li, Jin; Huang, Liyan……, Frequent alterations in cytoskeleton remodelling genes in primary and metastatic lung adenocarcinomas., Nature Communications, 6, p 10131, 2015/12/9.
17.Hou, Yong#; Wu, Kui#; Shi, Xulian#; Li, Fuqiang#; Song, Luting#; Wu, Hanjie#; Dean, Michael; Li, Guibo; Tsang, Shirley; Jiang, Runze; Zhang, Xiaolong; Li, Bo; Liu, Geng; Bedekar, Niharika; Lu, Na……, Comparison of variations detection between whole-genome amplification methods used in single-cell resequencing., GigaScience, 4, p 37, 2015.
18.Zhao, Libo#; Lu, Yi-Tsung#; Li, Fuqiang#; Wu, Kui; Hou, Shuang; Yu, Juehua; Shen, Qinglin; Wu, Dongxia; Song, Min; OuYang, Wei-Han; Luo, Zheng; Lee, Tom; Fang, Xiaohong; Shao, Chen; Xu, Xun……, High-purity prostate circulating tumor cell isolation by a polymer nanofiber-embedded microchip for whole exome sequencing., Advanced Materials, 25(21), pp 2897-2902, 2013/6/4.
19.Xiang R, Ma L, Yang M, Zheng Z, Chen X, Jia F, Xie F, Zhou Y, Li F, Wu K, Zhu Y. Increased expression of peptides from non-coding genes in cancer proteomics datasets suggests potential tumor neoantigens [J]. Commun Biol, 2021, 4(1): 496.
20.Huanzi Zhong, Yanqun Wang, Zhun Shi, Lu Zhang, Huahui Ren, Weiqun He, Zhaoyong Zhang, Airu Zhu, Jingxian Zhao, Fei Xiao, Fangming Yang, Tianzhu Liang, Feng Ye, Bei Zhong, Shicong Ruan, Mian Gan, Jiahui Zhu, Fang Li, Fuqiang Li, Daxi Wang, Jiandong Li, Peidi Ren, Shida Zhu, Huanming Yang, Jian Wang, Karsten Kristiansen, Hein Min Tun, Weijun Chen, Nanshan Zhong, Xun Xu, Yi-min Li, Junhua Li, Jincun Zhao, Characterization of respiratory microbial dysbiosis in hospitalized COVID-19 patients. Cell Discovery volume 7, Article number: 23 (2021)
21.Daxi Wang, Yanqun Wang, Wanying Sun, Lu Zhang, Jingkai Ji, Zhaoyong Zhang, Xinyi Cheng, Yimin Li, Fei Xiao, Airu Zhu, Bei Zhong, Shicong Ruan, Jiandong Li, Peidi Ren, Zhihua Ou, Minfeng Xiao, Min Li, Ziqing Deng, Huanzi Zhong, Fuqiang Li, Wenjing Wang, Yongwei Zhang, Weijun Chen, Shida Zhu, Xun Xu, Xin Jin, Jingxian Zhao, Nanshan Zhong, Wenwei Zhang, Jincun Zhao, Junhua Li, Yonghao Xu, Population Bottlenecks and Intra-host Evolution During Human-to-Human Transmission of SARS-CoV-2. Front. Med., 15 February 2021
22.Ponnapalli S P, Bradley M W, Devine K, Bowen J, Coppens S E, Leraas K M, Milash B A, Li F, Luo H, Qiu S, Wu K, Yang H, Wittwer C T, Palmer C A, Jensen R L, Gastier-Foster J M, Hanson H A, Barnholtz-Sloan J S, Alter O. Retrospective clinical trial experimentally validates glioblastoma genome-wide pattern of DNA copy-number alterations predictor of survival [J]. APL Bioeng, 2020, 4(2): 026106.
23.Dong L Q, Peng L H, Ma L J, Liu D B, Zhang S, Luo S Z, Rao J H, Zhu H W, Yang S X, Xi S J, Chen M, Xie F F, Li F Q, Li W H, Ye C, Lin L Y, Wang Y J, Wang X Y, Gao D M, Zhou H, Yang H M, Wang J, Zhu S D, Wang X D, Cao Y, Zhou J, Fan J, Wu K, Gao Q. Heterogeneous immunogenomic features and distinct escape mechanisms in multifocal hepatocellular carcinoma [J]. J Hepatol, 2020, 72(5): 896-908.
24.Wang C, Liang H, Lin C, Li F, Xie G, Qiao S, Shi X, Deng J, Zhao X, Wu K, Zhang X. Molecular Subtyping and Prognostic Assessment Based on Tumor Mutation Burden in Patients with Lung Adenocarcinomas [J]. Int J Mol Sci, 2019, 20(17):
25.Hong X, Qiao S, Li F, Wang W, Jiang R, Wu H, Chen H, Liu L, Peng J, Wang J, Jia C, Liang X, Dai H, Jiang J, Zhang T, Liao Q, Dai M, Cong L, Han X, Guo D, Liang Z, Li D, Zheng Z, Ye C, Li S, Zhao Y, Wu K, Wu W. Whole-genome sequencing reveals distinct genetic bases for insulinomas and non-functional pancreatic neuroendocrine tumours: leading to a new classification system [J]. Gut, 2020, 69(5): 877-887.
26.Zhang P, Kang B, Xie G, Li S, Gu Y, Shen Y, Zhao X, Ma Y, Li F, Si J, Wang J, Chen J, Yang H, Xu X, Yang Y. Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell lung cancer [J]. Cancer Lett, 2019, 442(53-67.
27.Shancheng Ren; Gong-Hong Wei; Dongbing Liu; Liguo Wang; Yong Hou; Shida Zhu; Lihua Peng; Qin Zhang; Yanbing Cheng; Hong Su; Xiuqing Zhou; Jibin Zhang; Fuqiang Li; Hancheng Zheng; Zhikun Zhao; Changjun Yin ……, Whole-genome and Transcriptome Sequencing of Prostate Cancer Identify New Genetic Alterations Driving Disease Progression. Eur Urol, 2017.
28.Pan, Qingfei; Lou, Xiaomin; Zhang, Ju; Zhu, Yinghui; Li, Fuqiang; Shan, Qiang; Chen, Xianwei; Xie, Yingying; Su, Siyuan; Wei, Hanfu; Lin, Liang; Wu, Lin; Liu, Siqi, Genomic variants in mouse model induced by azoxymethane and dextran sodium sulfate improperly mimic human colorectal cancer., Sci Rep, 7(1), p 25, 2017/12.
29.Zhao, Zhikun; Goldin, Lynn; Liu, Shiping; Wu, Liang; Zhou, Weiyin; Lou, Hong; Yu, Qichao; Tsang, Shirley X; Jiang, Miaomiao; Li, Fuqiang; McMaster, MaryLou; Li, Yang; Lin, Xinxin; Wang, Zhifeng……, Evolution of multiple cell clones over a 29-year period of a CLL patient., Nature Communications, 7(1), p 13765, 2016/12/16.
30.Xiao, Dakai; Pan, Hui; Li, Fuqiang; Wu, Kui; Zhang, Xin; He, Jianxing, Analysis of ultra-deep targeted sequencing reveals mutation burden is associated with gender and clinical outcome in lung adenocarcinoma., Oncotarget, 7(16), pp 22857-22864, 2016/4/19.
31.Jiang, Runze; Lu, Yi-Tsung; Ho, Hao; Li, Bo; Chen, Jie-Fu; Lin, Millicent; Li, Fuqiang; Wu, Kui; Wu, Hanjie; Lichterman, Jake; Wan, Haolei; Lu, Chia-Lun; OuYang, William; Ni, Ming; Wang, Linlin……, A comparison of isolated circulating tumor cells and tissue biopsies using whole-genome sequencing in prostate cancer., Oncotarget, 6(42), pp 44781-44793, 2015/12/29.
32.Wu, Liang; Zhang, Xiaolong; Zhao, Zhikun; Wang, Ling; Li, Bo; Li, Guibo; Dean, Michael; Yu, Qichao; Wang, Yanhui; Lin, Xinxin; Rao, Weijian; Mei, Zhanlong; Li, Yang; Jiang, Runze; Yang, Huan; Li Fuqiang……, Full-length single-cell RNA-seq applied to a viral human cancer: applications to HPV expression and splicing analysis in HeLa S3 cells., GigaScience, 4(1), p 51, 2015.
33.Zheng, Xiaoqi; Zhao, Qian; Wu, Hua-Jun; Li, Wei; Wang, Haiyun; Meyer, Clifford A; Qin, Qian Alvin; Xu, Han; Zang, Chongzhi; Jiang, Peng; Li, Fuqiang; Hou, Yong; He, Jianxing; Wang, Jun; Wang, Jun……, MethylPurify: tumor purity deconvolution and differential methylation detection from single tumor DNA methylomes., Genome Biology, 15(8), p 419, 2014/8/7.
34.Li, Yingrui; Xu, Xun; Song, Luting; Hou, Yong; Li, Zesong; Tsang, Shirley; Li, Fuqiang; Im, Kate McGee; Wu, Kui; Wu, Hanjie; Ye, Xiaofei; Li, Guibo; Wang, Linlin; Zhang, Bo; Liang, Jie; Xie, Wei……, Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer., GigaScience, 1(1), p 12, 2012/8/14.
35.Xu, Xun; Hou, Yong; Yin, Xuyang; Bao, Li; Tang, Aifa; Song, Luting; Li, Fuqiang; Tsang, Shirley; Wu, Kui; Wu, Hanjie; He, Weiming; Zeng, Liang; Xing, Manjie; Wu, Renhua; Jiang, Hui; Liu, Xiao……, Single-cell exome sequencing reveals single-nucleotide mutation characteristics of a kidney tumor., Cell, 148(5), pp 886-895, 2012/3/2.
36.Hou, Yong; Song, Luting; Zhu, Ping; Zhang, Bo; Tao, Ye; Xu, Xun; Li, Fuqiang; Wu, Kui; Liang, Jie; Shao, Di; Wu, Hanjie; Ye, Xiaofei; Ye, Chen; Wu, Renhua; Jian, Min; Chen, Yan; Xie, Wei……, Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm., Cell, 148(5), pp 873-885, 2012/3/2.
专利：
1.李甫强；周鑫兰；乔斯坦，评估肿瘤基因组测序数据分析质量的方法、装置及应用，2018/11/16，中国，CN111199776A
2.梁瀚 ；李甫强 ；吴逵 ；赵鑫 ；乔斯坦 ；周鑫兰， 确定待测样本类型的方法及系统，2018/6/11，中国，CN112739828A
3.梁瀚；李甫强；吴逵；赵鑫；乔斯坦；史旭莲，基于DNA测序数据的样本来源检测方法、装置和存储介质，2017/05/19, 中国，CN110462056A
4.吴逵；李甫强；侯勇；朱师达；叶晓飞；梁颜，肺腺癌生物标记物及其应用， 2015/11/5，中国，CN107849569A
5.吴逵；李甫强；侯勇；朱师达；叶晓飞；梁颜，BIOMARKER FOR DETECTION OF LUNG ADENOCARCINOMA AND USE THEREOF，韩国，10-2018-7015074
6.吴逵；李甫强；侯勇；朱师达；叶晓飞；梁颜，Biomarker for detection of lung adenocarcinoma and use thereof，欧洲，15907626.4
7.李甫强；谢国云；史旭莲；王杰；梁瀚；侯勇；吴逵，一种杂合性缺失的检测方法， 2015/1/30，中国，CN105986011B
8.吴汉杰；宋卢挺；侯勇；李甫强；李英睿；王俊；杨焕明，膀胱移行细胞癌易感性的相关基因及其预测方法和系统，2014/5/28，中国， CN103827313B
9.梁瀚; 李南南; 吴逵; 赵鑫; 李甫强; 林从; 罗甜，基于DNA甲基化水平确定样本类型的系统、可读介质及其应用，2022/05/31，中国，CN114566220A
10.李南南; 杨琴; 吴逵; 罗慧娟; 李甫强; 罗甜; 赵鑫，棒状杆菌作为尿液微生物标志物在制备用于检测膀胱癌的相关检测产品中的应用，2022/07/01，中国，CN114686607A
11.李南南; 杨琴; 吴逵; 林从; 李甫强; 罗甜; 罗慧娟; 赵鑫，尿液中DNA的提取方法、试剂盒及基因标志物在制备诊断膀胱癌的试剂盒中的应用，2023/04/18，中国，CN115976011A
12.罗慧娟; 罗甜; 李南南; 吴逵; 曹博洋; 李甫强; 李旺，固定平衡文库、其制备方法及提高基于亚硫酸盐处理文库测序质量的方法，2023/12/15，中国，CN117230535A
13.罗慧娟; 乔斯坦; 罗甜; 李南南; 曹博洋; 李甫强; 吴逵，测序文库的构建方法及试剂盒，2023/05/02，中国，CN116042767A
14.李甫强; 史旭莲; 谢国云; 鲁娜; 赵至坤; 蒋润泽; 梁瀚; 侯勇; 吴逵，CNV检测方法和装置，2016/10/05，中国，CN105986008A
