杜玉涛（duyt@genomics.cn）。丹麦奥胡斯大学人类遗传学博士，华大基因首席运营官，国家领军人才，中国科学院大学研究生导师，正高级。专长为家猪体外受精，手工克隆，玻璃化冷冻等技术的完善和优化，研究方向为手工克隆技术，辅助生殖技术以及基因组学。成功得到世界第一批手工克隆猪后代，后又将“手工克隆技术在家猪的应用”引进国内，研发出了中国首批“手工克隆猪”，效率达国际先进水平，2009年研发出世界首例“手工克隆绵羊”。成功研究出“迷你发光猪”、“克隆猪坚强”等前沿技术，完成了973项目“iPS克隆猪高效创建”，与中科院广州生物医药健康研究院等合作单位获得2015年广东省科学技术奖一等奖。带领团队研发了大规模测序的植入前筛查（PGS）技术，并于2012年与湖南中信湘雅生殖与遗传专科医院卢光琇团队合作陆续诞生了世界首批全基因组测序的试管婴儿。2017年完成农业部转基因重大专项“特异位点整合的安全高效转基因技术开发”课题。迄今她共主持/参与国家、省市科技项目17项，获得专利21项。在国际杂志发表高水平科研论文35篇，一作或通讯作者论文27篇。曾获深圳“双百计划”支持，获评广东省地方领军人物及国家级领军人才。
研究方向：基因组学医学应用
现任岗位：华大基因首席运营官
教育背景：丹麦奥胡斯大学人类遗传学博士
研究摘要
1.2008年研究家猪体外受精，手工克隆，玻璃化冷冻等技术的完善和优化，成功得到世界第一批手工克隆猪后代。
2.2008 年获得深圳“双百计划”支持，成功研究出“迷你发光猪”、“克隆猪坚强”。
3.2009 年研发出世界首例“手工克隆绵羊”。
4.大规模测序的植入前筛查（PGS）技术
5.宫颈癌相关研究
参与项目
1.项目“发育与生殖重要哺乳动物模型的建立”课题—小型猪基因组学研究, 参与, 国家级, 2011-01--2015-12
2.无标记定点整合动物转基因技术研究, 主持, 省级, 2012-11--2014-10
3.手工克隆小鼠项目研究, 主持, 省级, 2013-01--2015-01
4.手工克隆技术产业化推广项目, 主持, 省级, 2012-04--2014-03
5.超小型迷你猪转基因项目研究, 主持, 省级, 2011-02--2014-04
6.特异们点整合的安全高效转基因技术开发, 主持, 国家级, 2014-01--2016-12
7.干细胞与生殖细胞保存应用技术开发暨临床应用级（干）细胞库的建立, 主持, 省级, 2013-07--2015-07
8.基于Cas9-guid RNA共表达系统构建乳腺特异性敲除BRCA1基因小鼠的研究, 主持, 省级, 2014-12--2017-08
9.深圳动物基因组辅助育种工程实验室提升, 主持, 省级, 2016-07--2019-06
10.脑疾病患者多潜能干细胞模型的建立和应用研究, 主持, 省级, 2019-06--2022-06
论文
1.高会霞, 杨林, 郭云, 王逸丛, 刘玉珍, 唐岳, 赫章, 梁鑫明, 黄顺楷, 高鹏, 黄颖, 戴慕巍, 张志, 胡倩, 王瑜玲, 陈芳, 戴二黑, 江平, 杜玉涛. 石家庄新型冠状病毒肺炎疫情新型冠状病毒分子流行病学特征. 中华检验医学杂志[J]. 2022
2.Sjostedt, Evelina, Zhong, Wen, Fagerberg, Linn, Karlsson, Max, Mitsios, Nicholas, Adori, Csaba, Oksvold, Per, Edfors, Fredrik, Limiszewska, Agnieszka, Hikmet, Feria, Huang, Jinrong, Du, Yutao, Lin, Lin, Dong, Zhanying, Yang, Ling, Liu, Xin, Jiang, Hui, Xu, Xun, Wang, Jian, Yang, Huanming, Bolund, Lars, Mardinoglu, Adil, Zhang, Cheng, von Feilitzen, Kalle, Lindskog, Cecilia, Ponten, Fredrik, Luo, Yonglun, Hokfelt, Tomas, Uhlen, Mathias, Mulder, Jan. An atlas of the protein-coding genes in the human, pig, and mouse brain. SCIENCE[J]. 2020
3.Jian Liu, Xuemei Qiu, Daoming Wang, Yantao Li, Yang Zong, Yichen Liu, Yue Zhang, Ping Yang, Yong Zuo, Huanming Yang, Jian Wang, Yutao Du, Jin Zi. Quantification of 10 steroid hormones in human saliva from Chinese adult volunteers. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH[J]. 2018
4.Dong, Guoyi, Shang, Zhouchun, Liu, Longqi, Liu, Chuanyu, Ge, Yuping, Wang, Quanlei, Wu, Liang, Chen, Fang, Li, Baolin, Liu, Xin, Xu, Xun, Yang, Huanming, Du, Yutao, Jiang, Hui. Retinoic acid combined with spermatogonial stem cell conditions facilitate the generation of mouse germ-like cells. BIOSCIENCE REPORTS[J]. 2017
5.Yang, Zhenzhen, Vajta, Gabor, Xu, Ying, Luan, Jing, Lin, Mufei, Liu, Cong, Tian, Jianing, Dou, Hongwei, Li, Yong, Liu, Tianbin, Zhang, Yijie, Li, Lin, Yang, Wenxian, Bolund, Lars, Yang, Huanming, Du, Yutao. Production of Pigs by Hand-Made Cloning Using Mesenchymal Stem Cells and Fibroblasts. CELLULAR REPROGRAMMING[J]. 2016
6.Liu, Tianbin, Dou, Hongwei, Xiang, Xi, Li, Lin, Li, Yong, Lin, Lin, Pang, Xinzhi, Zhang, Yijie, Chen, Yu, Luan, Jing, Xu, Ying, Yang, Zhenzhen, Yang, Wenxian, Liu, Huan, Li, Feida, Wang, Hui, Yang, Huanming, Bolund, Lars, Vajta, Gabor, Du, Yutao. Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos. CELLULAR REPROGRAMMING[J]. 2015
7. Li, Feida, Li, Yong, Liu, Huan, Zhang, Xingju, Liu, Chuxin, Tian, Kai, Bolund, Lars, Dou, Hongwei, Yang, Wenxian, Yang, Huanming, Staunstrup, Nicklas Heine, Du, Yutao. Transgenic Wuzhishan minipigs designed to express a dominant-negative porcine growth hormone receptor display small stature and a perturbed insulin/IGF-1 pathway. TRANSGENIC RESEARCH[J]. 2015
8.李剑, Gábor, Vajta, 杜玉涛. 植入前遗传学诊断和筛查的最新进展. 中国产前诊断杂志（电子版）[J]. 2014
9.Tan, Yueqiu, Yin, Xuyang, Zhang, Shuoping, Jiang, Hui, Tan, Ke, Li, Jian, Xiong, Bo, Gong, Fei, Zhang, Chunlei, Pan, Xiaoyu, Chen, Fang, Chen, Shengpei, Gong, Chun, Lu, Changfu, Luo, Keli, Gu, Yifan, Zhang, Xiuqing, Wang, Wei, Xu, Xun, Vajta, Gabor, Bolund, Lars, Yang, Huanming, Lu, Guangxiu, Du, Yutao, Lin, Ge. Clinical outcome of preimplantation genetic diagnosis and screening using next generation sequencing. GIGASCIENCE[J]. 2014
10.Huan Liu, Yong Li, Qiang Wei, Chunxin Liu, Lars Bolund, Gbor Vajta, Hongwei Dou, Wenxian Yang, Ying Xu, Jing Luan, Jun Wang, Huanming Yang, Nicklas Heine Staunstrup, Yutao Du. Development of Transgenic Minipigs with Expression of Antimorphic Human Cryptochrome 1. PLOS ONE[J]. 2013
11.Zhang, Peng, Liu, Peng, Dou, Hongwei, Chen, Lei, Chen, Longxin, Lin, Lin, Tan, Pingping, Vajta, Gabor, Gao, Jianfeng, Du, Yutao, Ma, Runlin Z. Handmade Cloned Transgenic Sheep Rich in Omega-3 Fatty Acids. PLOS ONE[J]. 2013
12.Fan, Nana, Chen, Jijun, Shang, Zhouchun, Dou, Hongwei, Ji, Guangzhen, Zou, Qingjian, Wu, Lu, He, Lixiazi, Wang, Fang, Liu, Kai, Liu, Na, Han, Jianyong, Zhou, Qi, Pan, Dengke, Yang, Dongshan, Zhao, Bentian, Ouyang, Zhen, Liu, Zhaoming, Zhao, Yu, Lin, Lin, Zhong, Chongming, Wang, Quanlei, Wang, Shouqi, Xu, Ying, Luan, Jing, Liang, Yu, Yang, Zhenzhen, Li, Jing, Lu, Chunxia, Vajta, Gabor, Li, Ziyi, Ouyang, Hongsheng, Wang, Huayan, Wang, Yong, Yang, Yang, Liu, Zhonghua, Wei, Hong, Luan, Zhidong, Esteban, Miguel A, Deng, Hongkui, Yang, Huanming, Pei, Duanqing, Li, Ning, Pei, Gang, Liu, Lin, Du, Yutao, Xiao, Lei, Lai, Liangxue. Piglets cloned from induced pluripotent stem cells. CELL RESEARCH. 2013
13.Huan Liu, Yong Li, Qiang Wei, Chunxin Liu, Lars Bolund, Gbor Vajta, Hongwei Dou, Wenxian Yang, Ying Xu, Jing Luan, Jun Wang, Huanming Yang, Nicklas Heine Staunstrup, Yutao Du. Development of Transgenic Minipigs with Expression of Antimorphic Human Cryptochrome 1. PLOS ONE[J]. 2013
14.Zhang, Peng, Liu, Peng, Dou, Hongwei, Chen, Lei, Chen, Longxin, Lin, Lin, Tan, Pingping, Vajta, Gabor, Gao, Jianfeng, Du, Yutao, Ma, Runlin Z. Handmade Cloned Transgenic Sheep Rich in Omega-3 Fatty Acids. PLOS ONE[J]. 2013
15.Fan, Nana, Chen, Jijun, Shang, Zhouchun, Dou, Hongwei, Ji, Guangzhen, Zou, Qingjian, Wu, Lu, He, Lixiazi, Wang, Fang, Liu, Kai, Liu, Na, Han, Jianyong, Zhou, Qi, Pan, Dengke, Yang, Dongshan, Zhao, Bentian, Ouyang, Zhen, Liu, Zhaoming, Zhao, Yu, Lin, Lin, Zhong, Chongming, Wang, Quanlei, Wang, Shouqi, Xu, Ying, Luan, Jing, Liang, Yu, Yang, Zhenzhen, Li, Jing, Lu, Chunxia, Vajta, Gabor, Li, Ziyi, Ouyang, Hongsheng, Wang, Huayan, Wang, Yong, Yang, Yang, Liu, Zhonghua, Wei, Hong, Luan, Zhidong, Esteban, Miguel A, Deng, Hongkui, Yang, Huanming, Pei, Duanqing, Li, Ning, Pei, Gang, Liu, Lin, Du, Yutao, Xiao, Lei, Lai, Liangxue. Piglets cloned from induced pluripotent stem cells. CELL RESEARCH. 2013
16.Yutao Du, Songgang Li, Shutang Feng, Jian Wang, Huanming Yang, Lars Bolund, Jun Wang, Yingrui Li, Guojie Zhang, Liangxue Lai, Shulin Yang, Guangbiao Wang, Kui Li, Juan Wang, Chuxin Liu, Lijie Ren, Likai Mao, Dingding Fan, Huan Liu, Lan Yang, Zhiqiang Xiong, Xiaoqing Sun, Wei Zhao, Xuanting Jiang, Yuanxin Chen, Yue Feng, Yanfeng Zhang, Lijuan Han, Yong Li, Zhiyong Huang, Yulian Mou, Xiaodong Fang. The sequence and analysis of a Chinese pig genome. 2012
17.Cheng, Linzhao, Hansen, Nancy F, Zhao, Ling, Du, Yutao, Zou, Chunlin, Donovan, Frank X, Chou, BinKuan, Zhou, Guangyu, Li, Shijie, Dowey, Sarah N, Ye, Zhaohui, Chandrasekharappa, Settara C, Yang, Huanming, Mullikin, James C, Liu, P Paul, NISC Comparative Sequencing Progra. Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression. CELL STEM CELL[J]. 2012
18.Zhang, Peng, Zhang, Yidi, Dou, Hongwei, Yin, Jingdong, Chen, Yu, Pang, Xinzhi, Vajta, Gabor, Bolund, Lars, Du, Yutao, Ma, Runlin Z. Handmade Cloned Transgenic Piglets Expressing the Nematode Fat-1 Gene. CELLULAR REPROGRAMMING[J]. 2012
19.Zhang, Peng, Zhang, Yidi, Dou, Hongwei, Yin, Jingdong, Chen, Yu, Pang, Xinzhi, Vajta, Gabor, Bolund, Lars, Du, Yutao, Ma, Runlin Z. Handmade Cloned Transgenic Piglets Expressing the Nematode Fat-1 Gene. CELLULAR REPROGRAMMING[J]. 2012
20.Fei Gao, Yonglun Luo, Shengting Li, Jian Li, Lin Lin, Anders Lade Nielsen, Charlotte Brandt Srensen, Gbor Vajta, Jun Wang, Xiuqing Zhang, Yutao Du, Huanming Yang, Lars Bolund. Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls. PLOS ONE[J]. 2011
21.Luo, Yonglun, Li, Juan, Liu, Ying, Lin, Lin, Du, Yutao, Li, Shengting, Yang, Huanming, Vajta, Gabor, Callesen, Henrik, Bolund, Lars, Sorensen, Charlotte Brandt. High efficiency of BRCA1 knockout using rAAV-mediated gene targeting: developing a pig model for breast cancer. TRANSGENIC RESEARCH[J]. 2011
22.Lin, Lin, Pribenszky, Csaba, Molnar, Miklos, Kragh, Peter M, Du, Yutao, Zhang, Xiuqing, Yang, Huanming, Bolund, Lars, Callesen, Henrik, Machaty, Zoltan, Vajta, Gabor. High Hydrostatic Pressure Treatment of Porcine Oocytes Induces Parthenogenetic Activation. CELLULAR REPROGRAMMING[J]. 2010
23.Pribenszky, Csaba, Vajta, Gabor, Molnar, Miklos, Du, Yutao, Lin, Lin, Bolund, Lars, Yovich, John. Stress for Stress Tolerance? A Fundamentally New Approach in Mammalian Embryology. BIOLOGY OF REPRODUCTIONnull. 2010
24.Lin, Lin, Kragh, Peter M, Purup, Stig, Kuwayama, Masashige, Du, Yutao, Zhang, Xiuqing, Yang, Huanming, Bolund, Lars, Callesen, Henrik, Vajta, Gabor. Osmotic stress induced by sodium chloride, sucrose or trehalose improves cryotolerance and developmental competence of porcine oocytes. REPRODUCTION FERTILITY AND DEVELOPMENT[J]. 2009
25.Lin, Lin, Du, Yutao, Liu, Ying, Kragh, Peter M, Li, Juan, Purup, Stig, Kuwayama, Masashige, Zhang, Xiuqing, Yang, Huanming, Bolund, Lars, Vajta, Gabor. Elevated NaCl concentration improves cryotolerance and developmental competence of porcine oocytes. REPRODUCTIVE BIOMEDICINE ONLINE[J]. 2009
26.Du, Yutao, Lin, Lin, Schmidt, Mette, Bogh, Ingrid B, Kragh, Peter M, Sorensen, Charlotte B, Li, Juan, Purup, Stig, Pribenszky, Csaba, Molnar, Molnar, Kuwayama, Masaabc, Zhang, Xiuqing, Yang, Huanming, Bolund, Lars, Vajta, Gabor. High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. CLONING AND STEM CELLS[J]. 2008
27.Liu, Ying, Du, Yutao, Lin, Lin, Li, Juan, Kragh, Peter M, Kuwayama, Masashige, Bolund, Lars, Yang, Huanming, Vajta, Gabor. Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes. CRYOLETTERS[J]. 2008
28.Du, Y, Pribenszky, C S, Molnar, M, Zhang, X, Yang, H, Kuwayama, M, Pedersen, A M, Villemoes, K, Bolund, L, Vajta, G. High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification. REPRODUCTION[J]. 2008
29.Vajta, Gabor, Korosi, Tamas, Du, Yutao, Nakata, Kumiko, Ieda, Shoko, Kuwayama, Masashige, Nagy, Zsolt Peter. The Well-of-the-Well system: an efficient approach to improve embryo development. REPRODUCTIVE BIOMEDICINE ONLINE[J]. 2008
30.Du, Yutao, Lin, Lin, Schmidt, Mette, Bogh, Ingrid B, Kragh, Peter M, Sorensen, Charlotte B, Li, Juan, Purup, Stig, Pribenszky, Csaba, Molnar, Molnar, Kuwayama, Masaabc, Zhang, Xiuqing, Yang, Huanming, Bolund, Lars, Vajta, Gabor. High hydrostatic pressure treatment of porcine oocytes before handmade cloning improves developmental competence and cryosurvival. CLONING AND STEM CELLS[J]. 2008
31.Liu, Ying, Du, Yutao, Lin, Lin, Li, Juan, Kragh, Peter M, Kuwayama, Masashige, Bolund, Lars, Yang, Huanming, Vajta, Gabor. Comparison of efficiency of open pulled straw (OPS) and Cryotop vitrification for cryopreservation of in vitro matured pig oocytes. CRYOLETTERS[J]. 2008
32.Du, Y, Pribenszky, C S, Molnar, M, Zhang, X, Yang, H, Kuwayama, M, Pedersen, A M, Villemoes, K, Bolund, L, Vajta, G. High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification. REPRODUCTION[J]. 2008
33.Vajta, Gabor, Korosi, Tamas, Du, Yutao, Nakata, Kumiko, Ieda, Shoko, Kuwayama, Masashige, Nagy, Zsolt Peter. The Well-of-the-Well system: an efficient approach to improve embryo development. REPRODUCTIVE BIOMEDICINE ONLINE[J]. 2008
34.Du, Yutao, Li, Juan, Kragh, Peter M, Zhang, Yunhai, Schmidt, Mette, Bogh, Ingrid B, Zhang, Xiuqing, Purup, Stig, Kuwayama, M, Jorgensen, Arne L, Pedersen, Anette M, Villemoes, Klaus, Yang, Huanming, Bolund, Lars, Vajtai, Gabor. Piglets born from vitrified cloned blastocysts produced with a simplified method of delipation and nuclear transfer. CLONING AND STEM CELLS[J]. 2007
35.Du, Y, Kragh, P M, Zhang, Y, Li, J, Schmidt, M, Bogh, I B, Zhang, X, Purup, S, Jorgensen, A L, Pedersen, A M, Villemoes, K, Yang, H, Bolund, L, Vajta, G. Piglets born from handmade cloning, an innovative cloning method without micromanipulation. THERIOGENOLOGY[J]. 2007
36.Du, Y, Zhang, Y, Li, J, Kragh, P M, Kuwayama, M, Ieda, S, Zhang, X, Schmidt, M, Bogh, I B, Purup, S, Pedersen, A M, Villemoes, K, Yang, H, Bolund, L, Vajta, G. Simplified cryopreservation of porcine cloned blastocysts. CRYOBIOLOGY[J]. 2007
37.Kragh, PM, Du, Y, Corydon, TJ, Purup, S, Bolund, L, Vajta, G. Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activation. THERIOGENOLOGY[J]. 2005
38.Du, Y, Kragh, PM, Zhang, X, Purup, S, Yang, H, Bolund, L, Vajta, G. High overall in vitro efficiency of porcine handmade cloning (HMC) combining partial zona digestion and oocyte trisection with sequential culture. CLONING AND STEM CELLS[J]. 2005, 7(3): 199-205, [59] Du Yutao. Post-hatching development of the porcine and bovine embryo--defining criteria for expected development in vivo and in vitro. Theriogenology. 2005, [60] Kragh, PM, Du, Y, Corydon, TJ, Purup, S, Bolund, L, Vajta, G. Efficient in vitro production of porcine blastocysts by handmade cloning with a combined electrical and chemical activation. THERIOGENOLOGY[J]. 2005
39.Zhang, XQ, Yang, HM, Yu, J, Chen, C, Zhang, GY, Bao, JY, Du, YT, Kibukawa, M, Li, ZJ, Wang, J, Hu, SN, Dong, W, Wang, J, Gregersen, N, Niebuhr, E, Bolund, L. Genomic organization, transcript variants and comparative analysis of the human nucleoporin 155 (NUP155) gene. GENE[J]. 2002
专利
1.一种猪卵母细胞体外培养成熟方法, 发明, 2008, 第 5 作者, 专利号: ZL200910107266.5
2.一种细胞核移植方法, 发明, 2012, 第 1 作者, 专利号: CN 101580828 B
3.一种染色体标本滴片装置, 实用新型, 2011, 第 2 作者, 专利号: CN 101580828 B
4.Cell nuclear transfer, 发明, 2005, 第 1 作者, 专利号: P1094DK00
5. 一种用于胚胎或细胞的冷冻液及其用途, 发明, 2014, 第 2 作者, 专利号: CN 101580828 B
6.一种人工透明带及其制备方法和用途, 发明, 2014, 第 2 作者, 专利号: CN 102899283 B
7.转基因动物及其制备方法, 发明, 2015, 第 4 作者, 专利号: CN 102367454 B
8.猪假attp位点及其用途, 发明, 2014, 第 5 作者, 专利号: CN 102533741 B
9.一种突变型隐色素基因1和突变型隐色素基因1的转基因猪, 发明, 2015, 第 5 作者, 专利号: CN 103145824 B
10.构建体及其应用, 发明, 2015, 第 5 作者, 专利号: CN 103255168 B
11.封装胚胎的装置及方法, 发明, 2016, 第 2 作者, 专利号: CN 104206375 B
12.从血液中分离、培养细胞的方法及进行克隆非人动物的方法, 发明, 2018, 第 4 作者, 专利号: CN 104513807 B
13.制备克隆小型动物胚胎的方法, 发明, 2019, 第 5 作者, 专利号: CN 105087653 B
14.SNP标记及其应用, 发明, 2019, 第 5 作者, 专利号: CN 105821037 B
获奖情况
1.猪基因技术突变创新及基因修饰猪模型的建立, 一等奖, 省级, 2015
2.全国五一巾帼标兵, 国家级, 2013
3.广东省地方领军人才, 省级, 2009
4.深圳双百计划高端科技人才, 市地级, 2008
